Primer Details for TRINITY_DN22468_c0_g1_i1 | |||
|---|---|---|---|
| (T)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCGCTCCGTGGAAAACAGTA | AGAAGTTGTTTGTAGACAGATCCT | Start: 98 |
| Tm | 60.32 | 57.573 | End: 295 |
| Size | 20 | 24 | 198 |
| Primer 2 details | |||
| Sequence | AATGCCTCCTAATAGTTAGCTCAA | TACTGTTTTCCACGGAGCGG | Start: 13 |
| Tm | 57.499 | 60.32 | End: 117 |
| Size | 24 | 20 | 105 |
| Primer 3 details | |||
| Sequence | AAATGCCTCCTAATAGTTAGCTCA | TACTGTTTTCCACGGAGCGG | Start: 12 |
| Tm | 57.499 | 60.32 | End: 117 |
| Size | 24 | 20 | 106 |
| Primer 4 details | |||
| Sequence | CCGCTCCGTGGAAAACAGTA | AGTTGTTTGTAGACAGATCCTTTGA | Start: 98 |
| Tm | 60.32 | 58.408 | End: 292 |
| Size | 20 | 25 | 195 |
| Primer 5 details | |||
| Sequence | ATGCCTCCTAATAGTTAGCTCAAT | TACTGTTTTCCACGGAGCGG | Start: 14 |
| Tm | 57.306 | 60.32 | End: 117 |
| Size | 24 | 20 | 104 |
