ssr_primer
Primer Details for TRINITY_DN22417_c0_g1_i1 |
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| (A)15ggacaagcacatacaaagaaaggccagacctcaaaaaaaggcactcatgtttatgaaagtattgaaagtctgatatcaca(T)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAGGTAGAAAGGCCGTGACA | TCTGGCCTTTCTTTGTATGTGC | Start: 60 |
| Tm | 59.68 | 59.18 | End: 165 |
| Size | 20 | 22 | 106 |
| Primer 2 details |
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| Sequence | CAGGTAGAAAGGCCGTGACA | CTGGCCTTTCTTTGTATGTGCT | Start: 60 |
| Tm | 59.68 | 59.178 | End: 164 |
| Size | 20 | 22 | 105 |
| Primer 3 details |
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| Sequence | CAGGTAGAAAGGCCGTGACA | TGGCCTTTCTTTGTATGTGCT | Start: 60 |
| Tm | 59.68 | 58.125 | End: 163 |
| Size | 20 | 21 | 104 |
| Primer 4 details |
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| Sequence | GAAAGGCCGTGACATCCCAT | TCTGGCCTTTCTTTGTATGTGC | Start: 66 |
| Tm | 60.394 | 59.18 | End: 165 |
| Size | 20 | 22 | 100 |
| Primer 5 details |
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| Sequence | AATCGCAGGTAGAAAGGCCG | TCTGGCCTTTCTTTGTATGTGC | Start: 55 |
| Tm | 60.462 | 59.18 | End: 165 |
| Size | 20 | 22 | 111 |
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