Primer Details for TRINITY_DN22311_c0_g1_i1 | |||
|---|---|---|---|
| (A)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCTCCTTCCCAAATCACAAATT | GCCCTCCCCTCTTGTTCTTT | Start: 0 |
| Tm | 57.458 | 59.594 | End: 194 |
| Size | 22 | 20 | 195 |
| Primer 2 details | |||
| Sequence | GCTCCTTCCCAAATCACAAATT | TGCCCTCCCCTCTTGTTCTT | Start: 0 |
| Tm | 57.458 | 60.772 | End: 195 |
| Size | 22 | 20 | 196 |
| Primer 3 details | |||
| Sequence | GCTCCTTCCCAAATCACAAATTACA | GCCCTCCCCTCTTGTTCTTT | Start: 0 |
| Tm | 60.047 | 59.594 | End: 194 |
| Size | 25 | 20 | 195 |
| Primer 4 details | |||
| Sequence | GCTCCTTCCCAAATCACAAATT | GTGCCCTCCCCTCTTGTTC | Start: 0 |
| Tm | 57.458 | 60.002 | End: 196 |
| Size | 22 | 19 | 197 |
| Primer 5 details | |||
| Sequence | GCTCCTTCCCAAATCACAAATT | TGCCCTCCCCTCTTGTTCT | Start: 0 |
| Tm | 57.458 | 60.152 | End: 195 |
| Size | 22 | 19 | 196 |
