ssr_primer
Primer Details for TRINITY_DN221_c0_g1_i1 |
|---|
| (CTT)6: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGGCTTCTTCCCAGACCTTC | ACCTTCATTCCTTGGCTGCA | Start: 140 |
| Tm | 60.035 | 59.887 | End: 365 |
| Size | 20 | 20 | 226 |
| Primer 2 details |
|---|
| Sequence | GGGGAAGTCAAGGATGGCAA | ACCTTCATTCCTTGGCTGCA | Start: 98 |
| Tm | 59.96 | 59.887 | End: 365 |
| Size | 20 | 20 | 268 |
| Primer 3 details |
|---|
| Sequence | GGGCTTCTTCCCAGACCTTC | GGAGTAGAGGAAGCACTGCC | Start: 140 |
| Tm | 60.035 | 59.823 | End: 404 |
| Size | 20 | 20 | 265 |
| Primer 4 details |
|---|
| Sequence | GGCATATGGTTGGGGGAAGT | ACCTTCATTCCTTGGCTGCA | Start: 86 |
| Tm | 59.738 | 59.887 | End: 365 |
| Size | 20 | 20 | 280 |
| Primer 5 details |
|---|
| Sequence | GGGCTTCTTCCCAGACCTTC | CGATTGTTCCACAGGCGAAG | Start: 140 |
| Tm | 60.035 | 59.556 | End: 340 |
| Size | 20 | 20 | 201 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGGCTTCTTCCCAGACCTTC | ACCTTCATTCCTTGGCTGCA | Start: 140 |
| Tm | 60.035 | 59.887 | End: 365 |
| Size | 20 | 20 | 226 |
| Primer 2 details |
|---|
| Sequence | GGGGAAGTCAAGGATGGCAA | ACCTTCATTCCTTGGCTGCA | Start: 98 |
| Tm | 59.96 | 59.887 | End: 365 |
| Size | 20 | 20 | 268 |
| Primer 3 details |
|---|
| Sequence | GGGCTTCTTCCCAGACCTTC | GGAGTAGAGGAAGCACTGCC | Start: 140 |
| Tm | 60.035 | 59.823 | End: 404 |
| Size | 20 | 20 | 265 |
| Primer 4 details |
|---|
| Sequence | GGCATATGGTTGGGGGAAGT | ACCTTCATTCCTTGGCTGCA | Start: 86 |
| Tm | 59.738 | 59.887 | End: 365 |
| Size | 20 | 20 | 280 |
| Primer 5 details |
|---|
| Sequence | GGGCTTCTTCCCAGACCTTC | CGATTGTTCCACAGGCGAAG | Start: 140 |
| Tm | 60.035 | 59.556 | End: 340 |
| Size | 20 | 20 | 201 |
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