Primer Details for TRINITY_DN22101_c0_g1_i1 |
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| (A)13: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTCTTTTAGGTGTTGGACTCAA | TCTTGAGTTATCCATTCGAAACTCT | Start: 23 |
| Tm | 57.376 | 58.064 | End: 195 |
| Size | 23 | 25 | 173 |
| Primer 2 details |
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| Sequence | TTCTCTTTTAGGTGTTGGACTCA | TCTTGAGTTATCCATTCGAAACTCT | Start: 22 |
| Tm | 57.376 | 58.064 | End: 195 |
| Size | 23 | 25 | 174 |
| Primer 3 details |
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| Sequence | AGGTGTTGGACTCAAAATCTAACA | TCTTGAGTTATCCATTCGAAACTCT | Start: 31 |
| Tm | 58.309 | 58.064 | End: 195 |
| Size | 24 | 25 | 165 |
| Primer 4 details |
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| Sequence | TCTCTTTTAGGTGTTGGACTCAA | CCGACTTGTATTCATCTTGAGTTATCC | Start: 23 |
| Tm | 57.376 | 59.83 | End: 209 |
| Size | 23 | 27 | 187 |
| Primer 5 details |
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| Sequence | TTCTCTTTTAGGTGTTGGACTCA | CCGACTTGTATTCATCTTGAGTTATCC | Start: 22 |
| Tm | 57.376 | 59.83 | End: 209 |
| Size | 23 | 27 | 188 |
