Primer Details for TRINITY_DN22052_c0_g1_i1 | |||
|---|---|---|---|
| (A)12: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGTCCTTGAGAGTGAGAAGGGA | TTCTAGAGCAGGTTTTTGTAAGGC | Start: 54 |
| Tm | 59.889 | 59.24 | End: 153 |
| Size | 22 | 24 | 100 |
| Primer 2 details | |||
| Sequence | AAGTCCTTGAGAGTGAGAAGGG | TCTAGAGCAGGTTTTTGTAAGGCT | Start: 53 |
| Tm | 59.097 | 59.958 | End: 152 |
| Size | 22 | 24 | 100 |
| Primer 3 details | |||
| Sequence | AAGTCCTTGAGAGTGAGAAGGG | TCTAGAGCAGGTTTTTGTAAGGC | Start: 53 |
| Tm | 59.097 | 58.676 | End: 152 |
| Size | 22 | 23 | 100 |
| Primer 4 details | |||
| Sequence | AGTCCTTGAGAGTGAGAAGGGA | GGGAGAATTGTTTTTCTAGAGCAGG | Start: 54 |
| Tm | 59.889 | 59.875 | End: 166 |
| Size | 22 | 25 | 113 |
| Primer 5 details | |||
| Sequence | TCAAAGTCCTTGAGAGTGAGAAGG | AGAGCAGGTTTTTGTAAGGCT | Start: 50 |
| Tm | 59.96 | 57.77 | End: 149 |
| Size | 24 | 21 | 100 |
