Primer Details for TRINITY_DN21933_c0_g1_i1 |
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| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTTCTCTCTCTATTCATCACCACA | ACAGTTCGAACTAGGTTCAGAGT | Start: 89 |
| Tm | 58.809 | 59.11 | End: 234 |
| Size | 25 | 23 | 146 |
| Primer 2 details |
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| Sequence | CTCTTCTCTCTCTATTCATCACCACA | ACAGTTCGAACTAGGTTCAGAGT | Start: 88 |
| Tm | 59.679 | 59.11 | End: 234 |
| Size | 26 | 23 | 147 |
| Primer 3 details |
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| Sequence | ACAAAATGTGGTTTCTCTTCTCTCT | ACAGTTCGAACTAGGTTCAGAGT | Start: 74 |
| Tm | 58.639 | 59.11 | End: 234 |
| Size | 25 | 23 | 161 |
| Primer 4 details |
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| Sequence | TCTTCTCTCTCTATTCATCACCACA | TGCATGATGTTTAATAACCACCGG | Start: 89 |
| Tm | 58.809 | 59.903 | End: 266 |
| Size | 25 | 24 | 178 |
| Primer 5 details |
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| Sequence | AAATGTGGTTTCTCTTCTCTCTCT | ACAGTTCGAACTAGGTTCAGAGT | Start: 77 |
| Tm | 57.568 | 59.11 | End: 234 |
| Size | 24 | 23 | 158 |
