Primer Details for TRINITY_DN21896_c0_g1_i1 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTAGATCAGAAGGTATTAGCTGCA | ACTAGGAGCCTTGTGGTTCT | Start: 4 |
| Tm | 59.402 | 57.969 | End: 272 |
| Size | 25 | 20 | 269 |
| Primer 2 details |
|---|
| Sequence | AGATCAGAAGGTATTAGCTGCAT | ACTAGGAGCCTTGTGGTTCT | Start: 7 |
| Tm | 57.178 | 57.969 | End: 272 |
| Size | 23 | 20 | 266 |
| Primer 3 details |
|---|
| Sequence | TGTAGATCAGAAGGTATTAGCTGCA | ACTAGGAGCCTTGTGGTTCTT | Start: 4 |
| Tm | 59.402 | 58.66 | End: 272 |
| Size | 25 | 21 | 269 |
| Primer 4 details |
|---|
| Sequence | TGTAGATCAGAAGGTATTAGCTGCA | AACTAGGAGCCTTGTGGTTCT | Start: 4 |
| Tm | 59.402 | 58.66 | End: 273 |
| Size | 25 | 21 | 270 |
| Primer 5 details |
|---|
| Sequence | GTAGATCAGAAGGTATTAGCTGCA | ACTAGGAGCCTTGTGGTTCT | Start: 5 |
| Tm | 57.91 | 57.969 | End: 272 |
| Size | 24 | 20 | 268 |
