Primer Details for TRINITY_DN21628_c0_g1_i1 |
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| (TA)6: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGGAATCTGTAAATGTGAACATGG | TCTGTAAGATGTGATGGTGATACCA | Start: 20 |
| Tm | 59.581 | 59.339 | End: 158 |
| Size | 25 | 25 | 139 |
| Primer 2 details |
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| Sequence | GGGAATCTGTAAATGTGAACATGGT | TCTGTAAGATGTGATGGTGATACCA | Start: 21 |
| Tm | 59.581 | 59.339 | End: 158 |
| Size | 25 | 25 | 138 |
| Primer 3 details |
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| Sequence | TGGGAATCTGTAAATGTGAACATGG | CTGTAAGATGTGATGGTGATACCA | Start: 20 |
| Tm | 59.581 | 58.083 | End: 157 |
| Size | 25 | 24 | 138 |
| Primer 4 details |
|---|
| Sequence | GGGAATCTGTAAATGTGAACATGGT | CTGTAAGATGTGATGGTGATACCA | Start: 21 |
| Tm | 59.581 | 58.083 | End: 157 |
| Size | 25 | 24 | 137 |
| Primer 5 details |
|---|
| Sequence | GGGAATCTGTAAATGTGAACATGG | TCTGTAAGATGTGATGGTGATACCA | Start: 21 |
| Tm | 58.105 | 59.339 | End: 158 |
| Size | 24 | 25 | 138 |
