Primer Details for TRINITY_DN21597_c0_g1_i1 |
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| (A)16: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTGACTCGCCAACTTGAGTT | CCAATGATCTTCTTGGTTAAGCTGT | Start: 175 |
| Tm | 59.587 | 59.583 | End: 331 |
| Size | 21 | 25 | 157 |
| Primer 2 details |
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| Sequence | GCATACAAGTAGGTTTTGAAGAGGA | ACTCAAGTTGGCGAGTCAGA | Start: 89 |
| Tm | 59.062 | 58.959 | End: 194 |
| Size | 25 | 20 | 106 |
| Primer 3 details |
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| Sequence | AGCATACAAGTAGGTTTTGAAGAGG | ACTCAAGTTGGCGAGTCAGA | Start: 88 |
| Tm | 59.06 | 58.959 | End: 194 |
| Size | 25 | 20 | 107 |
| Primer 4 details |
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| Sequence | TCTGACTCGCCAACTTGAGTT | TCCAATGATCTTCTTGGTTAAGCTG | Start: 175 |
| Tm | 59.587 | 59.351 | End: 332 |
| Size | 21 | 25 | 158 |
| Primer 5 details |
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| Sequence | GCATACAAGTAGGTTTTGAAGAGG | ACTCAAGTTGGCGAGTCAGA | Start: 89 |
| Tm | 57.817 | 58.959 | End: 194 |
| Size | 24 | 20 | 106 |
