Primer Details for TRINITY_DN21574_c0_g1_i1 | |||
|---|---|---|---|
| (A)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTGTCGAATGAGTTTTAAACCCA | TGGGGCCAATGAAGAGTTGT | Start: 40 |
| Tm | 59.118 | 59.518 | End: 143 |
| Size | 24 | 20 | 104 |
| Primer 2 details | |||
| Sequence | GTGTCGAATGAGTTTTAAACCCA | TGGGGCCAATGAAGAGTTGT | Start: 41 |
| Tm | 57.573 | 59.518 | End: 143 |
| Size | 23 | 20 | 103 |
| Primer 3 details | |||
| Sequence | TGTGTCGAATGAGTTTTAAACCC | TGGGGCCAATGAAGAGTTGT | Start: 40 |
| Tm | 57.573 | 59.518 | End: 143 |
| Size | 23 | 20 | 104 |
| Primer 4 details | |||
| Sequence | TGTCGAATGAGTTTTAAACCCATGT | TGGGGCCAATGAAGAGTTGT | Start: 42 |
| Tm | 59.467 | 59.518 | End: 143 |
| Size | 25 | 20 | 102 |
| Primer 5 details | |||
| Sequence | TGTGTCGAATGAGTTTTAAACCCAT | TGGGGCCAATGAAGAGTTGT | Start: 40 |
| Tm | 59.467 | 59.518 | End: 143 |
| Size | 25 | 20 | 104 |
