ssr_primer
Primer Details for TRINITY_DN21548_c0_g1_i1 |
|---|
| (T)25: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCAATTGGGATGGAAAGGAGT | ATGGCCCATGAGTGAGCTTC | Start: 136 |
| Tm | 59.346 | 60.107 | End: 267 |
| Size | 22 | 20 | 132 |
| Primer 2 details |
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| Sequence | TCCAATTGGGATGGAAAGGAGT | AATGGCCCATGAGTGAGCTT | Start: 136 |
| Tm | 59.346 | 59.666 | End: 268 |
| Size | 22 | 20 | 133 |
| Primer 3 details |
|---|
| Sequence | TCCAATTGGGATGGAAAGGAGT | TGAGCTTCGGGCCTTGTTTA | Start: 136 |
| Tm | 59.346 | 59.601 | End: 255 |
| Size | 22 | 20 | 120 |
| Primer 4 details |
|---|
| Sequence | TCCAATTGGGATGGAAAGGAGTT | ATGGCCCATGAGTGAGCTTC | Start: 136 |
| Tm | 59.923 | 60.107 | End: 267 |
| Size | 23 | 20 | 132 |
| Primer 5 details |
|---|
| Sequence | CCAATTGGGATGGAAAGGAGT | ATGGCCCATGAGTGAGCTTC | Start: 137 |
| Tm | 57.894 | 60.107 | End: 267 |
| Size | 21 | 20 | 131 |
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