Primer Details for TRINITY_DN21250_c0_g1_i1 |
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| (A)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGCAGGATTTGGCAAGACAG | TGGAACTTTAGGCTTTTTGAAGGAC | Start: 121 |
| Tm | 59.83 | 59.931 | End: 237 |
| Size | 20 | 25 | 117 |
| Primer 2 details |
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| Sequence | CGCAGGATTTGGCAAGACAG | ACTTTAGGCTTTTTGAAGGACTGT | Start: 121 |
| Tm | 59.83 | 58.799 | End: 233 |
| Size | 20 | 24 | 113 |
| Primer 3 details |
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| Sequence | CGCAGGATTTGGCAAGACAG | TGGAACTTTAGGCTTTTTGAAGGA | Start: 121 |
| Tm | 59.83 | 58.795 | End: 237 |
| Size | 20 | 24 | 117 |
| Primer 4 details |
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| Sequence | CGCAGGATTTGGCAAGACAG | GGAACTTTAGGCTTTTTGAAGGACT | Start: 121 |
| Tm | 59.83 | 59.698 | End: 236 |
| Size | 20 | 25 | 116 |
| Primer 5 details |
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| Sequence | TGACCTCCCTAAAGTACTTTTCACA | CTGTCTTGCCAAATCCTGCG | Start: 13 |
| Tm | 59.633 | 59.83 | End: 140 |
| Size | 25 | 20 | 128 |
