Primer Details for TRINITY_DN21071_c0_g1_i1 | |||
|---|---|---|---|
| (A)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTCCTTAGTGTTTCTCGCCA | AGTTGACCCAAATATAGCAAGCT | Start: 6 |
| Tm | 58.755 | 58.14 | End: 173 |
| Size | 21 | 23 | 168 |
| Primer 2 details | |||
| Sequence | CTCCTTAGTGTTTCTCGCCA | AGTTGACCCAAATATAGCAAGCT | Start: 7 |
| Tm | 57.256 | 58.14 | End: 173 |
| Size | 20 | 23 | 167 |
| Primer 3 details | |||
| Sequence | TCTCCTTAGTGTTTCTCGCCAT | AGTTGACCCAAATATAGCAAGCT | Start: 6 |
| Tm | 59.168 | 58.14 | End: 173 |
| Size | 22 | 23 | 168 |
| Primer 4 details | |||
| Sequence | ATCTCCTTAGTGTTTCTCGCCA | AGTTGACCCAAATATAGCAAGCT | Start: 5 |
| Tm | 59.168 | 58.14 | End: 173 |
| Size | 22 | 23 | 169 |
| Primer 5 details | |||
| Sequence | TCTCCTTAGTGTTTCTCGCCA | TGACCCAAATATAGCAAGCTAGAGA | Start: 6 |
| Tm | 58.755 | 59.397 | End: 170 |
| Size | 21 | 25 | 165 |
