Primer Details for TRINITY_DN21056_c0_g1_i1 |
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| (T)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGTTCATAAGTGTATAAAGGGCGC | TGGATAGAAGTAGGAACAAACAGTCA | Start: 66 |
| Tm | 60.319 | 59.731 | End: 169 |
| Size | 24 | 26 | 104 |
| Primer 2 details |
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| Sequence | GCGTTCATAAGTGTATAAAGGGCG | TGGATAGAAGTAGGAACAAACAGTCA | Start: 65 |
| Tm | 60.319 | 59.731 | End: 169 |
| Size | 24 | 26 | 105 |
| Primer 3 details |
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| Sequence | AGAAAAGGTTTCTTCCCCATTTCT | GCAATAACACTATACCAAACTGCCA | Start: 11 |
| Tm | 58.532 | 59.875 | End: 121 |
| Size | 24 | 25 | 111 |
| Primer 4 details |
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| Sequence | TCATAAGTGTATAAAGGGCGCA | TGGATAGAAGTAGGAACAAACAGTCA | Start: 69 |
| Tm | 57.59 | 59.731 | End: 169 |
| Size | 22 | 26 | 101 |
| Primer 5 details |
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| Sequence | AGAAAAGGTTTCTTCCCCATTTCT | AGCAATAACACTATACCAAACTGCC | Start: 11 |
| Tm | 58.532 | 59.643 | End: 122 |
| Size | 24 | 25 | 112 |
