Primer Details for TRINITY_DN20845_c0_g1_i1 |
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| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAAACAAACGTACCTCCAATTCA | CTGAAGTTGATCGAAATTCTTTTCCG | Start: 144 |
| Tm | 57.161 | 59.479 | End: 243 |
| Size | 23 | 26 | 100 |
| Primer 2 details |
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| Sequence | AAAACAAACGTACCTCCAATTCAA | CTGAAGTTGATCGAAATTCTTTTCCG | Start: 144 |
| Tm | 57.79 | 59.479 | End: 243 |
| Size | 24 | 26 | 100 |
| Primer 3 details |
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| Sequence | TAAAACAAACGTACCTCCAATTCA | TGAAGTTGATCGAAATTCTTTTCCGA | Start: 143 |
| Tm | 57.061 | 59.796 | End: 242 |
| Size | 24 | 26 | 100 |
| Primer 4 details |
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| Sequence | AAAACAAACGTACCTCCAATTCAAA | CTGAAGTTGATCGAAATTCTTTTCCG | Start: 144 |
| Tm | 58.368 | 59.479 | End: 243 |
| Size | 25 | 26 | 100 |
| Primer 5 details |
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| Sequence | TAAAACAAACGTACCTCCAATTCA | TGAAGTTGATCGAAATTCTTTTCCG | Start: 143 |
| Tm | 57.061 | 58.629 | End: 242 |
| Size | 24 | 25 | 100 |
