Primer Details for TRINITY_DN20486_c0_g1_i1 |
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| (T)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTTTGAGACTGGCCATAGCG | AAATGTTTGTATCACCCTTCTCCT | Start: 77 |
| Tm | 60.676 | 58.108 | End: 176 |
| Size | 21 | 24 | 100 |
| Primer 2 details |
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| Sequence | TTTGAGACTGGCCATAGCGT | ACATAAATGTTTGTATCACCCTTCTCC | Start: 79 |
| Tm | 59.387 | 59.661 | End: 180 |
| Size | 20 | 27 | 102 |
| Primer 3 details |
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| Sequence | TGTTTGAGACTGGCCATAGCG | AAATGTTTGTATCACCCTTCTCCTT | Start: 77 |
| Tm | 60.676 | 58.685 | End: 176 |
| Size | 21 | 25 | 100 |
| Primer 4 details |
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| Sequence | TGTTTGAGACTGGCCATAGC | AAATGTTTGTATCACCCTTCTCCT | Start: 77 |
| Tm | 57.886 | 58.108 | End: 176 |
| Size | 20 | 24 | 100 |
| Primer 5 details |
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| Sequence | GTTTGAGACTGGCCATAGCG | TAAATGTTTGTATCACCCTTCTCCT | Start: 78 |
| Tm | 58.99 | 57.972 | End: 177 |
| Size | 20 | 25 | 100 |
