Primer Details for TRINITY_DN20189_c0_g1_i1 | |||
|---|---|---|---|
| (T)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCAGTCGGTATCTGTGGAGT | TCTTGGATTGAACCTATATGTCGGT | Start: 4 |
| Tm | 59.182 | 59.634 | End: 178 |
| Size | 20 | 25 | 175 |
| Primer 2 details | |||
| Sequence | GCAGTCGGTATCTGTGGAGT | TTTGTAAGCCATTTTGTAAGCCA | Start: 4 |
| Tm | 59.182 | 57.463 | End: 150 |
| Size | 20 | 23 | 147 |
| Primer 3 details | |||
| Sequence | GCAGTCGGTATCTGTGGAGT | CTTGGATTGAACCTATATGTCGGT | Start: 4 |
| Tm | 59.182 | 58.389 | End: 177 |
| Size | 20 | 24 | 174 |
| Primer 4 details | |||
| Sequence | GCAGTCGGTATCTGTGGAGT | TGGATTGAACCTATATGTCGGTT | Start: 4 |
| Tm | 59.182 | 57.376 | End: 175 |
| Size | 20 | 23 | 172 |
| Primer 5 details | |||
| Sequence | GCAGTCGGTATCTGTGGAGT | TTGGATTGAACCTATATGTCGGT | Start: 4 |
| Tm | 59.182 | 57.376 | End: 176 |
| Size | 20 | 23 | 173 |
