Primer Details for TRINITY_DN20132_c0_g1_i1 |
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| (A)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGTTCAAACTTAGTCTCCTTATTTTGA | GGAATTGCGAAATCAATGAGTAGACT | Start: 97 |
| Tm | 58.229 | 59.962 | End: 323 |
| Size | 27 | 26 | 227 |
| Primer 2 details |
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| Sequence | CGTTCAAACTTAGTCTCCTTATTTTGA | AGGAATTGCGAAATCAATGAGTAGAC | Start: 97 |
| Tm | 58.229 | 59.962 | End: 324 |
| Size | 27 | 26 | 228 |
| Primer 3 details |
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| Sequence | TCGTTCAAACTTAGTCTCCTTATTTTG | GGAATTGCGAAATCAATGAGTAGACT | Start: 96 |
| Tm | 58.229 | 59.962 | End: 323 |
| Size | 27 | 26 | 228 |
| Primer 4 details |
|---|
| Sequence | TCGTTCAAACTTAGTCTCCTTATTTTG | AGGAATTGCGAAATCAATGAGTAGAC | Start: 96 |
| Tm | 58.229 | 59.962 | End: 324 |
| Size | 27 | 26 | 229 |
| Primer 5 details |
|---|
| Sequence | CGTTCAAACTTAGTCTCCTTATTTTGA | AGGAATTGCGAAATCAATGAGTAGA | Start: 97 |
| Tm | 58.229 | 58.885 | End: 324 |
| Size | 27 | 25 | 228 |
