Primer Details for TRINITY_DN19885_c0_g1_i1 | |||
|---|---|---|---|
| (A)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCATTCAAGAAAAAGAAACTGCA | GTTTGTACTTTCGTTCGGCCA | Start: 23 |
| Tm | 58.582 | 59.401 | End: 135 |
| Size | 24 | 21 | 113 |
| Primer 2 details | |||
| Sequence | TGCATTCAAGAAAAAGAAACTGCA | AGTTTGTACTTTCGTTCGGCC | Start: 23 |
| Tm | 58.582 | 59.131 | End: 136 |
| Size | 24 | 21 | 114 |
| Primer 3 details | |||
| Sequence | TGCATTCAAGAAAAAGAAACTGCA | TGGTTTCAATCATGTCGCCA | Start: 23 |
| Tm | 58.582 | 58.097 | End: 195 |
| Size | 24 | 20 | 173 |
| Primer 4 details | |||
| Sequence | TGCATTCAAGAAAAAGAAACTGCA | TTTGTACTTTCGTTCGGCCA | Start: 23 |
| Tm | 58.582 | 58.049 | End: 134 |
| Size | 24 | 20 | 112 |
| Primer 5 details | |||
| Sequence | TGCATTCAAGAAAAAGAAACTGCAA | GTTTGTACTTTCGTTCGGCCA | Start: 23 |
| Tm | 59.126 | 59.401 | End: 135 |
| Size | 25 | 21 | 113 |
