Primer Details for TRINITY_DN19773_c0_g1_i1 |
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| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACAGAAACTTCAACAAAAAGAAGCA | GACGACATACAGGGGCTGTT | Start: 38 |
| Tm | 58.545 | 59.751 | End: 178 |
| Size | 25 | 20 | 141 |
| Primer 2 details |
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| Sequence | CATCATAATGCCTCCATTGATCAA | GACGACATACAGGGGCTGTT | Start: 61 |
| Tm | 57.416 | 59.751 | End: 178 |
| Size | 24 | 20 | 118 |
| Primer 3 details |
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| Sequence | ACAGAAACTTCAACAAAAAGAAGCA | AAGACGACATACAGGGGCTG | Start: 38 |
| Tm | 58.545 | 59.463 | End: 180 |
| Size | 25 | 20 | 143 |
| Primer 4 details |
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| Sequence | CATCATAATGCCTCCATTGATCAA | AAGACGACATACAGGGGCTG | Start: 61 |
| Tm | 57.416 | 59.463 | End: 180 |
| Size | 24 | 20 | 120 |
| Primer 5 details |
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| Sequence | ACAGAAACTTCAACAAAAAGAAGCA | ACGACATACAGGGGCTGTTT | Start: 38 |
| Tm | 58.545 | 59.309 | End: 177 |
| Size | 25 | 20 | 140 |
