ssr_primer
Primer Details for TRINITY_DN19499_c0_g1_i1 |
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| (AT)6: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTCAAGAACGCCAAGAGATGT | GTCAGAAGCGAGGTGAACCA | Start: 159 |
| Tm | 59.7 | 59.966 | End: 423 |
| Size | 22 | 20 | 265 |
| Primer 2 details |
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| Sequence | GTGTGGCTCTCCAAGATGCT | ACATCTCTTGGCGTTCTTGAGA | Start: 38 |
| Tm | 60.036 | 59.7 | End: 180 |
| Size | 20 | 22 | 143 |
| Primer 3 details |
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| Sequence | GGCTCTCCCACTCCAATAGC | ACATCTCTTGGCGTTCTTGAGA | Start: 7 |
| Tm | 59.892 | 59.7 | End: 180 |
| Size | 20 | 22 | 174 |
| Primer 4 details |
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| Sequence | GTTTGGCTCTCCCACTCCAA | ACATCTCTTGGCGTTCTTGAGA | Start: 3 |
| Tm | 59.89 | 59.7 | End: 180 |
| Size | 20 | 22 | 178 |
| Primer 5 details |
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| Sequence | ACGCCAAGAGATGTATTGCA | GTCAGAAGCGAGGTGAACCA | Start: 167 |
| Tm | 57.598 | 59.966 | End: 423 |
| Size | 20 | 20 | 257 |
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