Primer Details for TRINITY_DN19358_c0_g1_i1 |
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| (A)14(AAG)6*gagaagaataaaagtaaaaaagtttgatacatggttgagaaaatggtttcacaaagaagagtataata(ATG)6: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACATGGTTGAGAAAATGGTTTCACA | ACAAGATGGTTGCAGGGTGT | Start: 77 |
| Tm | 59.872 | 59.814 | End: 177 |
| Size | 25 | 20 | 101 |
| Primer 2 details |
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| Sequence | ACATGGTTGAGAAAATGGTTTCACA | CAAGATGGTTGCAGGGTGTG | Start: 77 |
| Tm | 59.872 | 59.4 | End: 176 |
| Size | 25 | 20 | 100 |
| Primer 3 details |
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| Sequence | CATGGTTGAGAAAATGGTTTCACA | ACAAGATGGTTGCAGGGTGT | Start: 78 |
| Tm | 58.4 | 59.814 | End: 177 |
| Size | 24 | 20 | 100 |
| Primer 4 details |
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| Sequence | ACATGGTTGAGAAAATGGTTTCAC | ACAAGATGGTTGCAGGGTGT | Start: 77 |
| Tm | 58.399 | 59.814 | End: 177 |
| Size | 24 | 20 | 101 |
| Primer 5 details |
|---|
| Sequence | ACATGGTTGAGAAAATGGTTTCA | ACAAGATGGTTGCAGGGTGT | Start: 77 |
| Tm | 57.132 | 59.814 | End: 177 |
| Size | 23 | 20 | 101 |
(T)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACATGGTTGAGAAAATGGTTTCACA | ACAAGATGGTTGCAGGGTGT | Start: 77 |
| Tm | 59.872 | 59.814 | End: 177 |
| Size | 25 | 20 | 101 |
| Primer 2 details |
|---|
| Sequence | ACATGGTTGAGAAAATGGTTTCACA | CAAGATGGTTGCAGGGTGTG | Start: 77 |
| Tm | 59.872 | 59.4 | End: 176 |
| Size | 25 | 20 | 100 |
| Primer 3 details |
|---|
| Sequence | CATGGTTGAGAAAATGGTTTCACA | ACAAGATGGTTGCAGGGTGT | Start: 78 |
| Tm | 58.4 | 59.814 | End: 177 |
| Size | 24 | 20 | 100 |
| Primer 4 details |
|---|
| Sequence | ACATGGTTGAGAAAATGGTTTCAC | ACAAGATGGTTGCAGGGTGT | Start: 77 |
| Tm | 58.399 | 59.814 | End: 177 |
| Size | 24 | 20 | 101 |
| Primer 5 details |
|---|
| Sequence | ACATGGTTGAGAAAATGGTTTCA | ACAAGATGGTTGCAGGGTGT | Start: 77 |
| Tm | 57.132 | 59.814 | End: 177 |
| Size | 23 | 20 | 101 |
