ssr_primer
Primer Details for TRINITY_DN1900_c0_g1_i1 |
|---|
| (TTG)6: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCGCGTAGAATCGTTGGTTC | TCGTGCTCCGGTTTCATCAA | Start: 889 |
| Tm | 59.975 | 59.966 | End: 1126 |
| Size | 20 | 20 | 238 |
| Primer 2 details |
|---|
| Sequence | TTGCCACCTTCCACTCGATC | CACTTGGGGAGATGGCCATT | Start: 1500 |
| Tm | 60.036 | 60.033 | End: 1616 |
| Size | 20 | 20 | 117 |
| Primer 3 details |
|---|
| Sequence | CCCCAACGGACGTAGAAACA | GATCGAGTGGAAGGTGGCAA | Start: 1294 |
| Tm | 59.966 | 60.036 | End: 1519 |
| Size | 20 | 20 | 226 |
| Primer 4 details |
|---|
| Sequence | ACCCCAACGGACGTAGAAAC | GATCGAGTGGAAGGTGGCAA | Start: 1293 |
| Tm | 59.966 | 60.036 | End: 1519 |
| Size | 20 | 20 | 227 |
| Primer 5 details |
|---|
| Sequence | TAAAGGCCATTGTCCGCTGT | CACTTGGGGAGATGGCCATT | Start: 1374 |
| Tm | 59.962 | 60.033 | End: 1616 |
| Size | 20 | 20 | 243 |
(CCA)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCGCGTAGAATCGTTGGTTC | TCGTGCTCCGGTTTCATCAA | Start: 889 |
| Tm | 59.975 | 59.966 | End: 1126 |
| Size | 20 | 20 | 238 |
| Primer 2 details |
|---|
| Sequence | TTGCCACCTTCCACTCGATC | CACTTGGGGAGATGGCCATT | Start: 1500 |
| Tm | 60.036 | 60.033 | End: 1616 |
| Size | 20 | 20 | 117 |
| Primer 3 details |
|---|
| Sequence | CCCCAACGGACGTAGAAACA | GATCGAGTGGAAGGTGGCAA | Start: 1294 |
| Tm | 59.966 | 60.036 | End: 1519 |
| Size | 20 | 20 | 226 |
| Primer 4 details |
|---|
| Sequence | ACCCCAACGGACGTAGAAAC | GATCGAGTGGAAGGTGGCAA | Start: 1293 |
| Tm | 59.966 | 60.036 | End: 1519 |
| Size | 20 | 20 | 227 |
| Primer 5 details |
|---|
| Sequence | TAAAGGCCATTGTCCGCTGT | CACTTGGGGAGATGGCCATT | Start: 1374 |
| Tm | 59.962 | 60.033 | End: 1616 |
| Size | 20 | 20 | 243 |
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