Primer Details for TRINITY_DN18950_c0_g1_i1 | |||
|---|---|---|---|
| (T)12: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTTCAAAAGAACAACCCTCCA | TGTGTGTTTGAGAACATTTGTTGT | Start: 146 |
| Tm | 57.756 | 58.222 | End: 373 |
| Size | 22 | 24 | 228 |
| Primer 2 details | |||
| Sequence | TGGAAAAACAGTAGACTTCAACCA | TGGGTGCACAATCAATTACTTTCT | Start: 90 |
| Tm | 58.498 | 59.168 | End: 231 |
| Size | 24 | 24 | 142 |
| Primer 3 details | |||
| Sequence | TCTTCAAAAGAACAACCCTCCAA | TGTGTGTTTGAGAACATTTGTTGT | Start: 146 |
| Tm | 58.395 | 58.222 | End: 373 |
| Size | 23 | 24 | 228 |
| Primer 4 details | |||
| Sequence | TTCTTCAAAAGAACAACCCTCCA | TGTGTGTTTGAGAACATTTGTTGT | Start: 145 |
| Tm | 58.395 | 58.222 | End: 373 |
| Size | 23 | 24 | 229 |
| Primer 5 details | |||
| Sequence | TGGAAAAACAGTAGACTTCAACCA | GGGTGCACAATCAATTACTTTCTCA | Start: 90 |
| Tm | 58.498 | 60.048 | End: 230 |
| Size | 24 | 25 | 141 |
