Primer Details for TRINITY_DN18775_c0_g1_i1 |
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| (ATAAT)5: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTACTTGTAGTGATTTATTTGCTGGT | CGCCTGTTCCGTTCCATGTA | Start: 74 |
| Tm | 58.899 | 60.391 | End: 291 |
| Size | 27 | 20 | 218 |
| Primer 2 details |
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| Sequence | TCTACTTGTAGTGATTTATTTGCTGGT | TACGCCTGTTCCGTTCCATG | Start: 74 |
| Tm | 58.899 | 60.391 | End: 293 |
| Size | 27 | 20 | 220 |
| Primer 3 details |
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| Sequence | TCTACTTGTAGTGATTTATTTGCTGGT | ATACGCCTGTTCCGTTCCAT | Start: 74 |
| Tm | 58.899 | 59.46 | End: 294 |
| Size | 27 | 20 | 221 |
| Primer 4 details |
|---|
| Sequence | CTACTTGTAGTGATTTATTTGCTGGT | CGCCTGTTCCGTTCCATGTA | Start: 75 |
| Tm | 57.731 | 60.391 | End: 291 |
| Size | 26 | 20 | 217 |
| Primer 5 details |
|---|
| Sequence | CTACTTGTAGTGATTTATTTGCTGGT | TACGCCTGTTCCGTTCCATG | Start: 75 |
| Tm | 57.731 | 60.391 | End: 293 |
| Size | 26 | 20 | 219 |
