Primer Details for TRINITY_DN18695_c0_g1_i1 |
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| (AT)6: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACACACACAATGATGGGATACT | AGTGCTAATAAAATCCCATGTTTGGT | Start: 68 |
| Tm | 57.425 | 59.56 | End: 247 |
| Size | 22 | 26 | 180 |
| Primer 2 details |
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| Sequence | ACACACACAATGATGGGATACT | GTGCTAATAAAATCCCATGTTTGGT | Start: 68 |
| Tm | 57.425 | 58.36 | End: 246 |
| Size | 22 | 25 | 179 |
| Primer 3 details |
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| Sequence | ACACACACAATGATGGGATACT | TGCTAATAAAATCCCATGTTTGGT | Start: 68 |
| Tm | 57.425 | 57.134 | End: 245 |
| Size | 22 | 24 | 178 |
| Primer 4 details |
|---|
| Sequence | ACACACACAATGATGGGATACT | AGTGCTAATAAAATCCCATGTTTGG | Start: 68 |
| Tm | 57.425 | 58.123 | End: 247 |
| Size | 22 | 25 | 180 |
| Primer 5 details |
|---|
| Sequence | ACACACACAATGATGGGATACT | AGTGCTAATAAAATCCCATGTTTGGTT | Start: 68 |
| Tm | 57.425 | 60.038 | End: 247 |
| Size | 22 | 27 | 180 |
