Primer Details for TRINITY_DN18642_c0_g1_i1 |
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| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCTCAGATTGCATGGGCTAG | TGAATCCACTTTGTTTAAACTTGGA | Start: 190 |
| Tm | 59.858 | 57.589 | End: 289 |
| Size | 21 | 25 | 100 |
| Primer 2 details |
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| Sequence | ACCTCAGATTGCATGGGCTAG | TCTAGGCCTGTAGACATATCAAAAA | Start: 190 |
| Tm | 59.858 | 57.568 | End: 459 |
| Size | 21 | 25 | 270 |
| Primer 3 details |
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| Sequence | ACCTCAGATTGCATGGGCTAG | TTCTAGGCCTGTAGACATATCAAAA | Start: 190 |
| Tm | 59.858 | 57.568 | End: 460 |
| Size | 21 | 25 | 271 |
| Primer 4 details |
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| Sequence | ACCTCAGATTGCATGGGCTAG | TTTCTAGGCCTGTAGACATATCAAA | Start: 190 |
| Tm | 59.858 | 57.568 | End: 461 |
| Size | 21 | 25 | 272 |
| Primer 5 details |
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| Sequence | ACCTCAGATTGCATGGGCTAG | TTTTCTAGGCCTGTAGACATATCAA | Start: 190 |
| Tm | 59.858 | 57.568 | End: 462 |
| Size | 21 | 25 | 273 |
