Primer Details for TRINITY_DN18589_c0_g1_i1 |
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| (A)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGCTACTACATAAGAAAACAAAAAGGG | TGAAAAATTATTTATGCAATAGCCGTG | Start: 86 |
| Tm | 58.973 | 57.971 | End: 189 |
| Size | 27 | 27 | 104 |
| Primer 2 details |
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| Sequence | TGGCTACTACATAAGAAAACAAAAAGG | AATTATTTATGCAATAGCCGTGAAAGA | Start: 85 |
| Tm | 58.427 | 58.371 | End: 184 |
| Size | 27 | 27 | 100 |
| Primer 3 details |
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| Sequence | GGCTACTACATAAGAAAACAAAAAGGG | GAAAAATTATTTATGCAATAGCCGTGA | Start: 86 |
| Tm | 58.973 | 57.756 | End: 188 |
| Size | 27 | 27 | 103 |
| Primer 4 details |
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| Sequence | GGCTACTACATAAGAAAACAAAAAGGG | AAATTATTTATGCAATAGCCGTGAAAG | Start: 86 |
| Tm | 58.973 | 57.752 | End: 185 |
| Size | 27 | 27 | 100 |
| Primer 5 details |
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| Sequence | TGGCTACTACATAAGAAAACAAAAAGG | AATTATTTATGCAATAGCCGTGAAAG | Start: 85 |
| Tm | 58.427 | 57.198 | End: 184 |
| Size | 27 | 26 | 100 |
