Primer Details for TRINITY_DN18305_c0_g1_i1 |
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| (T)22g(T)24: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGAGAGGTGTGAGAGATGGT | ACGAAGGAAAGGTCAACTAATTAAACA | Start: 123 |
| Tm | 57.388 | 59.5 | End: 242 |
| Size | 20 | 27 | 120 |
| Primer 2 details |
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| Sequence | TGAGAGGTGTGAGAGATGGT | CTAACGAAGGAAAGGTCAACTAATT | Start: 123 |
| Tm | 57.388 | 57.331 | End: 245 |
| Size | 20 | 25 | 123 |
| Primer 3 details |
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| Sequence | TGAGAGGTGTGAGAGATGGTT | ACGAAGGAAAGGTCAACTAATTAAACA | Start: 123 |
| Tm | 58.105 | 59.5 | End: 242 |
| Size | 21 | 27 | 120 |
| Primer 4 details |
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| Sequence | TTGAGAGGTGTGAGAGATGGT | ACGAAGGAAAGGTCAACTAATTAAACA | Start: 122 |
| Tm | 58.105 | 59.5 | End: 242 |
| Size | 21 | 27 | 121 |
| Primer 5 details |
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| Sequence | AGAGGTGTGAGAGATGGTTTGT | ACGAAGGAAAGGTCAACTAATTAAACA | Start: 125 |
| Tm | 59.025 | 59.5 | End: 242 |
| Size | 22 | 27 | 118 |
