Primer Details for TRINITY_DN18290_c0_g1_i1 | |||
|---|---|---|---|
| (T)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTCAAATTGGTGAAATCAACACA | AGAGAAGGCATGTGACGGTC | Start: 25 |
| Tm | 58.029 | 59.752 | End: 195 |
| Size | 24 | 20 | 171 |
| Primer 2 details | |||
| Sequence | TGTCAAATTGGTGAAATCAACACA | GAGAGAAGGCATGTGACGGT | Start: 25 |
| Tm | 58.029 | 59.752 | End: 196 |
| Size | 24 | 20 | 172 |
| Primer 3 details | |||
| Sequence | TGTCAAATTGGTGAAATCAACACA | GCATGTGACGGTCCGTGTAT | Start: 25 |
| Tm | 58.029 | 60.46 | End: 188 |
| Size | 24 | 20 | 164 |
| Primer 4 details | |||
| Sequence | TGTCAAATTGGTGAAATCAACACA | AGAGAGAAGGCATGTGACGG | Start: 25 |
| Tm | 58.029 | 59.466 | End: 197 |
| Size | 24 | 20 | 173 |
| Primer 5 details | |||
| Sequence | TGGACCTTTTTCCAATCTTTTTAGCT | AGAGAAGGCATGTGACGGTC | Start: 76 |
| Tm | 59.674 | 59.752 | End: 195 |
| Size | 26 | 20 | 120 |
