Primer Details for TRINITY_DN18230_c0_g1_i1 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCTTTTACAAACTCTGGATCTCT | TGACTCTAATGTTTGTCAGAACACT | Start: 18 |
| Tm | 57.559 | 58.417 | End: 147 |
| Size | 24 | 25 | 130 |
| Primer 2 details |
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| Sequence | ACAACTACCTTTTACAAACTCTGGA | TGACTCTAATGTTTGTCAGAACACT | Start: 12 |
| Tm | 58.344 | 58.417 | End: 147 |
| Size | 25 | 25 | 136 |
| Primer 3 details |
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| Sequence | ACAAACTCTGGATCTCTTGACTAA | TGACTCTAATGTTTGTCAGAACACT | Start: 25 |
| Tm | 57.328 | 58.417 | End: 147 |
| Size | 24 | 25 | 123 |
| Primer 4 details |
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| Sequence | TTACAAACTCTGGATCTCTTGACT | TGACTCTAATGTTTGTCAGAACACT | Start: 23 |
| Tm | 57.328 | 58.417 | End: 147 |
| Size | 24 | 25 | 125 |
| Primer 5 details |
|---|
| Sequence | ACCTTTTACAAACTCTGGATCTCT | TGACTCTAATGTTTGTCAGAACAC | Start: 18 |
| Tm | 57.559 | 57.155 | End: 147 |
| Size | 24 | 24 | 130 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCTTTTACAAACTCTGGATCTCT | TGACTCTAATGTTTGTCAGAACACT | Start: 18 |
| Tm | 57.559 | 58.417 | End: 147 |
| Size | 24 | 25 | 130 |
| Primer 2 details |
|---|
| Sequence | ACAACTACCTTTTACAAACTCTGGA | TGACTCTAATGTTTGTCAGAACACT | Start: 12 |
| Tm | 58.344 | 58.417 | End: 147 |
| Size | 25 | 25 | 136 |
| Primer 3 details |
|---|
| Sequence | ACAAACTCTGGATCTCTTGACTAA | TGACTCTAATGTTTGTCAGAACACT | Start: 25 |
| Tm | 57.328 | 58.417 | End: 147 |
| Size | 24 | 25 | 123 |
| Primer 4 details |
|---|
| Sequence | TTACAAACTCTGGATCTCTTGACT | TGACTCTAATGTTTGTCAGAACACT | Start: 23 |
| Tm | 57.328 | 58.417 | End: 147 |
| Size | 24 | 25 | 125 |
| Primer 5 details |
|---|
| Sequence | ACCTTTTACAAACTCTGGATCTCT | TGACTCTAATGTTTGTCAGAACAC | Start: 18 |
| Tm | 57.559 | 57.155 | End: 147 |
| Size | 24 | 24 | 130 |
