Primer Details for TRINITY_DN18228_c0_g1_i1 | |||
|---|---|---|---|
| (A)12: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATGGTTCGTTGTGGTGCTTG | TGGCCTGTATCATCTCATATCAAT | Start: 117 |
| Tm | 59.332 | 57.368 | End: 255 |
| Size | 20 | 24 | 139 |
| Primer 2 details | |||
| Sequence | TGGTTCGTTGTGGTGCTTGT | TGGCCTGTATCATCTCATATCAAT | Start: 118 |
| Tm | 60.678 | 57.368 | End: 255 |
| Size | 20 | 24 | 138 |
| Primer 3 details | |||
| Sequence | ATGGTTCGTTGTGGTGCTTG | TGGCCTGTATCATCTCATATCAATT | Start: 117 |
| Tm | 59.332 | 57.976 | End: 255 |
| Size | 20 | 25 | 139 |
| Primer 4 details | |||
| Sequence | AATGGTTCGTTGTGGTGCTTG | TGGCCTGTATCATCTCATATCAAT | Start: 116 |
| Tm | 59.933 | 57.368 | End: 255 |
| Size | 21 | 24 | 140 |
| Primer 5 details | |||
| Sequence | TGGTTCGTTGTGGTGCTTGT | TGGCCTGTATCATCTCATATCAATT | Start: 118 |
| Tm | 60.678 | 57.976 | End: 255 |
| Size | 20 | 25 | 138 |
