ssr_primer
Primer Details for TRINITY_DN18176_c0_g1_i1 |
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| (A)13: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGTTTTTCATCCCCGGCCT | TGTTTGTAGGACTGTGTCGAACA | Start: 300 |
| Tm | 60.179 | 60.119 | End: 485 |
| Size | 20 | 23 | 186 |
| Primer 2 details |
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| Sequence | CTTGTTTTTCATCCCCGGCC | TGTTTGTAGGACTGTGTCGAACA | Start: 299 |
| Tm | 59.754 | 60.119 | End: 485 |
| Size | 20 | 23 | 187 |
| Primer 3 details |
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| Sequence | ATCCCCGGCCTATTTGCATC | TGTTTGTAGGACTGTGTCGAACA | Start: 309 |
| Tm | 60.251 | 60.119 | End: 485 |
| Size | 20 | 23 | 177 |
| Primer 4 details |
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| Sequence | TTCATCCCCGGCCTATTTGC | TGTTTGTAGGACTGTGTCGAACA | Start: 306 |
| Tm | 60.467 | 60.119 | End: 485 |
| Size | 20 | 23 | 180 |
| Primer 5 details |
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| Sequence | CCCCGGCCTATTTGCATCTT | TGTTTGTAGGACTGTGTCGAACA | Start: 311 |
| Tm | 60.468 | 60.119 | End: 485 |
| Size | 20 | 23 | 175 |
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