Primer Details for TRINITY_DN18160_c0_g1_i1 | |||
|---|---|---|---|
| (T)16: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCTTGATAGACGAATAGGAGTGT | GGGTTCAAGTCCCGGCAA | Start: 85 |
| Tm | 58.553 | 59.886 | End: 221 |
| Size | 24 | 18 | 137 |
| Primer 2 details | |||
| Sequence | TCCTTGATAGACGAATAGGAGTGT | GGGTTCAAGTCCCGGCAAC | Start: 85 |
| Tm | 58.553 | 61.266 | End: 221 |
| Size | 24 | 19 | 137 |
| Primer 3 details | |||
| Sequence | CCTTGATAGACGAATAGGAGTGT | GGGTTCAAGTCCCGGCAA | Start: 86 |
| Tm | 57.224 | 59.886 | End: 221 |
| Size | 23 | 18 | 136 |
| Primer 4 details | |||
| Sequence | CCTTGATAGACGAATAGGAGTGT | GGGTTCAAGTCCCGGCAAC | Start: 86 |
| Tm | 57.224 | 61.266 | End: 221 |
| Size | 23 | 19 | 136 |
| Primer 5 details | |||
| Sequence | ATCCTTGATAGACGAATAGGAGTGT | GGGTTCAAGTCCCGGCAA | Start: 84 |
| Tm | 58.928 | 59.886 | End: 221 |
| Size | 25 | 18 | 138 |
