Primer Details for TRINITY_DN18088_c0_g1_i1 |
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| (AT)8: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCAGGACCTTTCCCTTAGGGT | TGTTCAACAAAGGAAGTATAAAGCA | Start: 97 |
| Tm | 59.846 | 57.425 | End: 237 |
| Size | 21 | 25 | 141 |
| Primer 2 details |
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| Sequence | TCAGGACCTTTCCCTTAGGGT | TGGTGTGTTAAATGTTACCAAAAACT | Start: 97 |
| Tm | 59.846 | 58.387 | End: 285 |
| Size | 21 | 26 | 189 |
| Primer 3 details |
|---|
| Sequence | TCAGGACCTTTCCCTTAGGGT | TGGTGTGTTAAATGTTACCAAAAAC | Start: 97 |
| Tm | 59.846 | 57.171 | End: 285 |
| Size | 21 | 25 | 189 |
| Primer 4 details |
|---|
| Sequence | TCAGGACCTTTCCCTTAGGGT | TGTTACCAAAAACTTTTCATTCAACCA | Start: 97 |
| Tm | 59.846 | 59.122 | End: 273 |
| Size | 21 | 27 | 177 |
| Primer 5 details |
|---|
| Sequence | TCAGGACCTTTCCCTTAGGGT | ACCAAAAACTTTTCATTCAACCAAT | Start: 97 |
| Tm | 59.846 | 57.064 | End: 269 |
| Size | 21 | 25 | 173 |
