ssr_primer
Primer Details for TRINITY_DN18052_c0_g1_i1 |
|---|
| (AGA)5: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGACGGATTTGACGAGGACG | ATTCCCCCTTCTCTCTCCCC | Start: 549 |
| Tm | 60.109 | 60.03 | End: 656 |
| Size | 20 | 20 | 108 |
| Primer 2 details |
|---|
| Sequence | TTGAGATGGAATAGGGCGGC | CATCGTCCTCTCCCAACTCG | Start: 364 |
| Tm | 59.891 | 59.899 | End: 616 |
| Size | 20 | 20 | 253 |
| Primer 3 details |
|---|
| Sequence | TTGAGATGGAATAGGGCGGC | CAATCCCTCCCCCATGGTTC | Start: 364 |
| Tm | 59.891 | 60.106 | End: 637 |
| Size | 20 | 20 | 274 |
| Primer 4 details |
|---|
| Sequence | TTGAGATGGAATAGGGCGGC | CGTCCTCGTCAAATCCGTCA | Start: 364 |
| Tm | 59.891 | 60.109 | End: 568 |
| Size | 20 | 20 | 205 |
| Primer 5 details |
|---|
| Sequence | AGCTGCTGCAGAACATACGA | ATTCCCCCTTCTCTCTCCCC | Start: 402 |
| Tm | 59.753 | 60.03 | End: 656 |
| Size | 20 | 20 | 255 |
(GA)9: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGACGGATTTGACGAGGACG | ATTCCCCCTTCTCTCTCCCC | Start: 549 |
| Tm | 60.109 | 60.03 | End: 656 |
| Size | 20 | 20 | 108 |
| Primer 2 details |
|---|
| Sequence | TTGAGATGGAATAGGGCGGC | CATCGTCCTCTCCCAACTCG | Start: 364 |
| Tm | 59.891 | 59.899 | End: 616 |
| Size | 20 | 20 | 253 |
| Primer 3 details |
|---|
| Sequence | TTGAGATGGAATAGGGCGGC | CAATCCCTCCCCCATGGTTC | Start: 364 |
| Tm | 59.891 | 60.106 | End: 637 |
| Size | 20 | 20 | 274 |
| Primer 4 details |
|---|
| Sequence | TTGAGATGGAATAGGGCGGC | CGTCCTCGTCAAATCCGTCA | Start: 364 |
| Tm | 59.891 | 60.109 | End: 568 |
| Size | 20 | 20 | 205 |
| Primer 5 details |
|---|
| Sequence | AGCTGCTGCAGAACATACGA | ATTCCCCCTTCTCTCTCCCC | Start: 402 |
| Tm | 59.753 | 60.03 | End: 656 |
| Size | 20 | 20 | 255 |
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