Primer Details for TRINITY_DN1802_c0_g1_i1 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCAAACTCTAAGGGTTCGT | TCAAGAAACAAGAAACAGAAACAGT | Start: 0 |
| Tm | 57.077 | 57.786 | End: 144 |
| Size | 20 | 25 | 145 |
| Primer 2 details |
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| Sequence | TGCAAACTCTAAGGGTTCGT | ACAAGAAACAGAAACAGTAACAGTT | Start: 0 |
| Tm | 57.077 | 57.551 | End: 137 |
| Size | 20 | 25 | 138 |
| Primer 3 details |
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| Sequence | TGCAAACTCTAAGGGTTCGT | AACAAGAAACAGAAACAGTAACAGT | Start: 0 |
| Tm | 57.077 | 57.551 | End: 138 |
| Size | 20 | 25 | 139 |
| Primer 4 details |
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| Sequence | TGCAAACTCTAAGGGTTCGTT | TCAAGAAACAAGAAACAGAAACAGT | Start: 0 |
| Tm | 57.797 | 57.786 | End: 144 |
| Size | 21 | 25 | 145 |
| Primer 5 details |
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| Sequence | GCAAACTCTAAGGGTTCGTTTGG | TCAAGAAACAAGAAACAGAAACAGT | Start: 1 |
| Tm | 60.304 | 57.786 | End: 144 |
| Size | 23 | 25 | 144 |
