ssr_primer
Primer Details for TRINITY_DN17739_c0_g1_i1 |
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| (AT)6: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCATCAAAGGCTTCCCCAAC | GAGGTGAAGAGAGAAGCTGCA | Start: 133 |
| Tm | 59.753 | 59.725 | End: 255 |
| Size | 20 | 21 | 123 |
| Primer 2 details |
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| Sequence | GCATCAAAGGCTTCCCCAAC | AGGTGAAGAGAGAAGCTGCA | Start: 133 |
| Tm | 59.753 | 58.654 | End: 254 |
| Size | 20 | 20 | 122 |
| Primer 3 details |
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| Sequence | GCATCAAAGGCTTCCCCAAC | AGAGGTGAAGAGAGAAGCTGC | Start: 133 |
| Tm | 59.753 | 59.45 | End: 256 |
| Size | 20 | 21 | 124 |
| Primer 4 details |
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| Sequence | GGGCTGACAATGTTGGGGAA | GAGGTGAAGAGAGAAGCTGCA | Start: 66 |
| Tm | 60.542 | 59.725 | End: 255 |
| Size | 20 | 21 | 190 |
| Primer 5 details |
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| Sequence | GGGCTGACAATGTTGGGGAA | AGGTGAAGAGAGAAGCTGCA | Start: 66 |
| Tm | 60.542 | 58.654 | End: 254 |
| Size | 20 | 20 | 189 |
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