Primer Details for TRINITY_DN172_c0_g1_i1 |
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| (T)13: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGGTGCACGTGAAATCTTTGA | ACATATGACGAAAGAAAGGGAGA | Start: 120 |
| Tm | 59.737 | 57.323 | End: 230 |
| Size | 21 | 23 | 111 |
| Primer 2 details |
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| Sequence | AGGAAAGAGTATGGTTTGATGGTGA | GGTTCTATTTCCCTAGCTCGCA | Start: 67 |
| Tm | 59.986 | 59.897 | End: 167 |
| Size | 25 | 22 | 101 |
| Primer 3 details |
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| Sequence | AGGAAAGAGTATGGTTTGATGGTGA | TGGTTCTATTTCCCTAGCTCGC | Start: 67 |
| Tm | 59.986 | 59.897 | End: 168 |
| Size | 25 | 22 | 102 |
| Primer 4 details |
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| Sequence | CGGTGCACGTGAAATCTTTGA | TGACGAAAGAAAGGGAGAAAATAGG | Start: 120 |
| Tm | 59.737 | 59.062 | End: 225 |
| Size | 21 | 25 | 106 |
| Primer 5 details |
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| Sequence | CGGTGCACGTGAAATCTTTGA | ACATATGACGAAAGAAAGGGAGAA | Start: 120 |
| Tm | 59.737 | 57.95 | End: 230 |
| Size | 21 | 24 | 111 |
