Primer Details for TRINITY_DN16836_c0_g1_i1 | |||
|---|---|---|---|
| (T)12: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGAAAAAGCCAGATAGTTGCT | AGAACAAGGTGCACCTCGTT | Start: 24 |
| Tm | 57.96 | 59.82 | End: 250 |
| Size | 22 | 20 | 227 |
| Primer 2 details | |||
| Sequence | AAGGAAAAAGCCAGATAGTTGCT | AGAACAAGGTGCACCTCGTT | Start: 23 |
| Tm | 58.589 | 59.82 | End: 250 |
| Size | 23 | 20 | 228 |
| Primer 3 details | |||
| Sequence | AAGGAAAAAGCCAGATAGTTGC | AGAACAAGGTGCACCTCGTT | Start: 23 |
| Tm | 57.209 | 59.82 | End: 250 |
| Size | 22 | 20 | 228 |
| Primer 4 details | |||
| Sequence | AGGAAAAAGCCAGATAGTTGCTA | AGAACAAGGTGCACCTCGTT | Start: 24 |
| Tm | 57.821 | 59.82 | End: 250 |
| Size | 23 | 20 | 227 |
| Primer 5 details | |||
| Sequence | TGTAAGGAAAAAGCCAGATAGTTGC | AGAACAAGGTGCACCTCGTT | Start: 20 |
| Tm | 59.817 | 59.82 | End: 250 |
| Size | 25 | 20 | 231 |
