Primer Details for TRINITY_DN16578_c0_g1_i1 | |||
|---|---|---|---|
| (C)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGACCCATATGACCTCATCTCT | GGTCCTCACCCATGTTAAGCT | Start: 128 |
| Tm | 57.795 | 59.718 | End: 403 |
| Size | 22 | 21 | 276 |
| Primer 2 details | |||
| Sequence | ACTCTTGGGTAAGATTCTCAAGGT | TTATTTGAGGTGGGTGGGGG | Start: 332 |
| Tm | 59.153 | 59.292 | End: 447 |
| Size | 24 | 20 | 116 |
| Primer 3 details | |||
| Sequence | ACTCTTGGGTAAGATTCTCAAGGT | TTTATTTGAGGTGGGTGGGGG | Start: 332 |
| Tm | 59.153 | 59.922 | End: 448 |
| Size | 24 | 21 | 117 |
| Primer 4 details | |||
| Sequence | TGACCCATATGACCTCATCTCT | GGTCCTCACCCATGTTAAGC | Start: 128 |
| Tm | 57.795 | 58.249 | End: 403 |
| Size | 22 | 20 | 276 |
| Primer 5 details | |||
| Sequence | ACTCTTGGGTAAGATTCTCAAGGTT | TTATTTGAGGTGGGTGGGGG | Start: 332 |
| Tm | 59.689 | 59.292 | End: 447 |
| Size | 25 | 20 | 116 |
