Primer Details for TRINITY_DN16463_c0_g1_i1 |
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| (T)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCAAATAACTGAAAAACGGCCT | TGCATTTCAAAGAAAAGAAAGTGCC | Start: 171 |
| Tm | 59.36 | 59.706 | End: 283 |
| Size | 23 | 25 | 113 |
| Primer 2 details |
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| Sequence | CACCAAATAACTGAAAAACGGCC | TGCATTTCAAAGAAAAGAAAGTGCC | Start: 170 |
| Tm | 59.261 | 59.706 | End: 283 |
| Size | 23 | 25 | 114 |
| Primer 3 details |
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| Sequence | CCACCAAATAACTGAAAAACGGC | TGCATTTCAAAGAAAAGAAAGTGCC | Start: 169 |
| Tm | 59.261 | 59.706 | End: 283 |
| Size | 23 | 25 | 115 |
| Primer 4 details |
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| Sequence | ACCAAATAACTGAAAAACGGCCT | GCATTTCAAAGAAAAGAAAGTGCCT | Start: 171 |
| Tm | 59.36 | 59.475 | End: 282 |
| Size | 23 | 25 | 112 |
| Primer 5 details |
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| Sequence | CACCAAATAACTGAAAAACGGCC | GCATTTCAAAGAAAAGAAAGTGCCT | Start: 170 |
| Tm | 59.261 | 59.475 | End: 282 |
| Size | 23 | 25 | 113 |
