Primer Details for TRINITY_DN16446_c0_g1_i1 | |||
|---|---|---|---|
| (T)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCCATTTTGGAAAGCTCTTTGC | GCAACATATCTACTCGAACCACT | Start: 77 |
| Tm | 59.518 | 58.316 | End: 294 |
| Size | 22 | 23 | 218 |
| Primer 2 details | |||
| Sequence | GCCATTTTGGAAAGCTCTTTGC | GCAACATATCTACTCGAACCACTT | Start: 77 |
| Tm | 59.518 | 58.89 | End: 294 |
| Size | 22 | 24 | 218 |
| Primer 3 details | |||
| Sequence | CCATTTTGGAAAGCTCTTTGCC | GCAACATATCTACTCGAACCACT | Start: 78 |
| Tm | 58.931 | 58.316 | End: 294 |
| Size | 22 | 23 | 217 |
| Primer 4 details | |||
| Sequence | CCATTTTGGAAAGCTCTTTGCCT | GCAACATATCTACTCGAACCACT | Start: 78 |
| Tm | 60.244 | 58.316 | End: 294 |
| Size | 23 | 23 | 217 |
| Primer 5 details | |||
| Sequence | GCCATTTTGGAAAGCTCTTTGC | GCAACATATCTACTCGAACCACTTT | Start: 77 |
| Tm | 59.518 | 59.419 | End: 294 |
| Size | 22 | 25 | 218 |
