Primer Details for TRINITY_DN161_c0_g1_i1 |
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| (A)15: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATCGGCGAGCTCAGAAGATG | TGGTTTTGACCTTTATGTTGTTTGA | Start: 53 |
| Tm | 59.968 | 58.064 | End: 190 |
| Size | 20 | 25 | 138 |
| Primer 2 details |
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| Sequence | CATCGGCGAGCTCAGAAGAT | TGGTTTTGACCTTTATGTTGTTTGA | Start: 52 |
| Tm | 59.968 | 58.064 | End: 190 |
| Size | 20 | 25 | 139 |
| Primer 3 details |
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| Sequence | TTCATCGGCGAGCTCAGAAG | TGGTTTTGACCTTTATGTTGTTTGA | Start: 50 |
| Tm | 60.179 | 58.064 | End: 190 |
| Size | 20 | 25 | 141 |
| Primer 4 details |
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| Sequence | ACCGTAAGTTCATCGGCGAG | TGGTTTTGACCTTTATGTTGTTTGA | Start: 42 |
| Tm | 60.179 | 58.064 | End: 190 |
| Size | 20 | 25 | 149 |
| Primer 5 details |
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| Sequence | ATCGGCGAGCTCAGAAGATG | TGTTTTGGTTTTGACCTTTATGTTGT | Start: 53 |
| Tm | 59.968 | 58.838 | End: 195 |
| Size | 20 | 26 | 143 |
