Primer Details for TRINITY_DN16146_c0_g1_i1 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACAACATCACCATAAGAGAGGAAGA | TGTTTGATTTGGATTCTACTCCATTT | Start: 91 |
| Tm | 59.515 | 57.579 | End: 204 |
| Size | 25 | 26 | 114 |
| Primer 2 details |
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| Sequence | ACAACATCACCATAAGAGAGGAAGA | ATGTTTGATTTGGATTCTACTCCATT | Start: 91 |
| Tm | 59.515 | 57.4 | End: 205 |
| Size | 25 | 26 | 115 |
| Primer 3 details |
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| Sequence | ACAACATCACCATAAGAGAGGAAG | TGTTTGATTTGGATTCTACTCCATTT | Start: 91 |
| Tm | 58.261 | 57.579 | End: 204 |
| Size | 24 | 26 | 114 |
| Primer 4 details |
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| Sequence | ACAACATCACCATAAGAGAGGAA | TGTTTGATTTGGATTCTACTCCATTT | Start: 91 |
| Tm | 57.238 | 57.579 | End: 204 |
| Size | 23 | 26 | 114 |
| Primer 5 details |
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| Sequence | AACAACATCACCATAAGAGAGGA | TGTTTGATTTGGATTCTACTCCATTT | Start: 90 |
| Tm | 57.238 | 57.579 | End: 204 |
| Size | 23 | 26 | 115 |
