ssr_primer
Primer Details for TRINITY_DN16081_c0_g1_i1 |
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| (ACC)5: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AACTCCAACAACCGAGGCAA | AGCCAGGGATGTTGCTGTTT | Start: 23 |
| Tm | 60.107 | 60.179 | End: 194 |
| Size | 20 | 20 | 172 |
| Primer 2 details |
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| Sequence | CAACCACCACCACAACAACC | AGCCAGGGATGTTGCTGTTT | Start: 95 |
| Tm | 59.825 | 60.179 | End: 194 |
| Size | 20 | 20 | 100 |
| Primer 3 details |
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| Sequence | AACTCCAACAACCGAGGCAA | CTAGGTGGTGGTGGTGGTTG | Start: 23 |
| Tm | 60.107 | 60.251 | End: 129 |
| Size | 20 | 20 | 107 |
| Primer 4 details |
|---|
| Sequence | AACTCCAACAACCGAGGCAA | GGTTCCGAGCAGGGCTATAG | Start: 23 |
| Tm | 60.107 | 59.681 | End: 149 |
| Size | 20 | 20 | 127 |
| Primer 5 details |
|---|
| Sequence | AACTCCAACAACCGAGGCAA | GTTCCGAGCAGGGCTATAGC | Start: 23 |
| Tm | 60.107 | 60.32 | End: 148 |
| Size | 20 | 20 | 126 |
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