Primer Details for TRINITY_DN15991_c0_g1_i1 | |||
|---|---|---|---|
| (T)13: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCAGTGATAGACGATAAACACGA | ACAACAATTGTGGGAATGATGACA | Start: 55 |
| Tm | 57.194 | 59.412 | End: 257 |
| Size | 23 | 24 | 203 |
| Primer 2 details | |||
| Sequence | AATTGTCCATTGAACAGAACGAT | ACAACAATTGTGGGAATGATGACA | Start: 132 |
| Tm | 57.035 | 59.412 | End: 257 |
| Size | 23 | 24 | 126 |
| Primer 3 details | |||
| Sequence | TCAGTGATAGACGATAAACACGA | TGTGGGAATGATGACAAGAATCA | Start: 55 |
| Tm | 57.194 | 58.079 | End: 249 |
| Size | 23 | 23 | 195 |
| Primer 4 details | |||
| Sequence | TCAGTGATAGACGATAAACACGAA | ACAACAATTGTGGGAATGATGACA | Start: 55 |
| Tm | 57.814 | 59.412 | End: 257 |
| Size | 24 | 24 | 203 |
| Primer 5 details | |||
| Sequence | TCAGTGATAGACGATAAACACGA | AGACTATCGTTCTGTTCAATGGACA | Start: 55 |
| Tm | 57.194 | 60.047 | End: 159 |
| Size | 23 | 25 | 105 |
