Primer Details for TRINITY_DN15947_c0_g1_i1 | |||
|---|---|---|---|
| (A)12: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCTTTTTGTGCCCTACTGTCC | CCACGAGTAGTTTACAATTGGACA | Start: 63 |
| Tm | 59.73 | 59.008 | End: 190 |
| Size | 21 | 24 | 128 |
| Primer 2 details | |||
| Sequence | GCTTTTTGTGCCCTACTGTCC | ACCACGAGTAGTTTACAATTGGAC | Start: 63 |
| Tm | 59.73 | 59.007 | End: 191 |
| Size | 21 | 24 | 129 |
| Primer 3 details | |||
| Sequence | GCTTTTTGTGCCCTACTGTCC | CCACGAGTAGTTTACAATTGGACAC | Start: 63 |
| Tm | 59.73 | 60.107 | End: 190 |
| Size | 21 | 25 | 128 |
| Primer 4 details | |||
| Sequence | GCTTTTTGTGCCCTACTGTCC | ACCACGAGTAGTTTACAATTGGA | Start: 63 |
| Tm | 59.73 | 57.782 | End: 191 |
| Size | 21 | 23 | 129 |
| Primer 5 details | |||
| Sequence | GCTTTTTGTGCCCTACTGTCC | ACCACGAGTAGTTTACAATTGGACA | Start: 63 |
| Tm | 59.73 | 60.454 | End: 191 |
| Size | 21 | 25 | 129 |
