Primer Details for TRINITY_DN15818_c0_g1_i1 | |||
|---|---|---|---|
| (T)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTTTGTTCCTATTACGACTCTCT | TGCAATCTATGTGCATCTTTGGA | Start: 80 |
| Tm | 57.414 | 58.728 | End: 250 |
| Size | 24 | 23 | 171 |
| Primer 2 details | |||
| Sequence | TGTTTGTTCCTATTACGACTCTCT | TGCAATCTATGTGCATCTTTGG | Start: 80 |
| Tm | 57.414 | 57.358 | End: 250 |
| Size | 24 | 22 | 171 |
| Primer 3 details | |||
| Sequence | TGTTTGTTCCTATTACGACTCTCTT | TGCAATCTATGTGCATCTTTGGA | Start: 80 |
| Tm | 58.01 | 58.728 | End: 250 |
| Size | 25 | 23 | 171 |
| Primer 4 details | |||
| Sequence | TTGTTTGTTCCTATTACGACTCTCT | TGCAATCTATGTGCATCTTTGGA | Start: 79 |
| Tm | 58.01 | 58.728 | End: 250 |
| Size | 25 | 23 | 172 |
| Primer 5 details | |||
| Sequence | TGTTTGTTCCTATTACGACTCTCT | TGCAATCTATGTGCATCTTTGGAA | Start: 80 |
| Tm | 57.414 | 59.294 | End: 250 |
| Size | 24 | 24 | 171 |
